Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Serum (a) and bronchoalveolar lavage (BAL) fluid (b) adiponectin concentrations in wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized to ovalbumin (OVA) and challenged with aerosols of OVA or PBS every day for 3 days. Data shown are mean ± SEM of 5–7 mice per group. # P < 0.05 versus WT mice with the same exposure.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Transgenic Assay

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Leukocytes in BAL fluid (a) and blood (b) and BAL IL-13 (c), BAL eotaxin (d), and serum OVA-specific IgE (e) of wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) and exposed to aerosols of OVA or PBS. Data shown are mean ± SEM of 5-6 PBS and 9-10 OVA treated mice per group except for OVA-specific IgE ( n = 5-6 for PBS and 12-13 for OVA). * P < 0.05 versus PBS challenged mice of the same genotype; # P < 0.05 versus WT mice with the same exposure. MAC: macrophages; NEU: neutrophils; EOS: eosinophils; LYM: lymphocytes; MON: mononuclear cells.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Transgenic Assay

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Pulmonary mRNA expression of Retnla (a), Itln1 (b), Alox15 (c), Clca3 (d), Agr2 (e), Muc5ac (f), Cxcl9 (g), and Il17a (h) in wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) and exposed to aerosols of OVA or PBS every day for 3 days. Data shown are the means ± SEM of 5-6 mice/group. Data are normalized relative to 18S expression. * P < 0.05 versus PBS challenged mice of the same genotype; # P < 0.05 versus WT mice with the same challenge.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Expressing, Transgenic Assay

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Leukocytes in BAL fluid (a) and blood (b), and BAL IL-13 (c), BAL eotaxin (d), and serum OVA-specific IgE (e) in wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) and challenged i.n. with OVA or PBS every week for 4 weeks. Data shown are mean ± SEM of 5–10 mice per group. * P < 0.05 versus PBS challenged mice of the same genotype; # P < 0.05 versus WT mice with the same challenge. MAC: macrophages; NEU: neutrophils; EOS: eosinophils; LYM: lymphocytes; MON: mononuclear cells.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Transgenic Assay

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Histological sections of airways of wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) without alum and challenged intranasally with OVA or PBS every week for 4 weeks (chronic). Light micrographs showing H&E (panels (a)–(d)) or PAS (panels (e)–(h)) staining. Arrowheads in (a)–(d) indicate regions of inflammatory cell accumulation. Arrows in (e)–(h) indicate regions of “purple” staining that were recognized by Image Pro software (Olympus) during quantification of goblet cell abundance (see text). The bars in panels (a) and (e) = 10 μ m and correspond to all images in the upper and lower row, respectively. PAS staining for mucous-containing goblet cells ((e)–(h)) is from medium sized peripheral airways.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Transgenic Assay, Staining, Software

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Pulmonary mRNA expression of Retnla (a), Itln1 (b), Alox15 (c), Clca3 (d), Agr2 (e), Muc5ac (f), Cxcl9 (g), and Il17a (h) in wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) and challenged i.n. with OVA or PBS once a week for 4 weeks. Data shown are the means ± SEM of 6–11 mice/group. Data are normalized relative to 18S expression. * P < 0.05 versus PBS challenged mice of the same genotype.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Expressing, Transgenic Assay

Journal: Journal of Allergy

Article Title: Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

doi: 10.1155/2013/349520

Figure Lengend Snippet: Pulmonary mRNA expression of Chi3l3 (a) and Clec10a (b) in wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) and challenged i.n. with OVA or PBS. Data shown are the means ± SEM of 6–11 mice/group. * P < 0.05 versus PBS challenged mice of the same genotype. (c) Expression of Chi3l3 , Clec10a , and Retnla mRNA in alveolar macrophages harvested from naïve wildtype and Adipo Tg mice. Results are mean ± SEM of data from 5–8 mice in each group. Data were normalized relative to 18S expression.

Article Snippet: The first, an acute challenge protocol, was similar to that used in a previous study in which we examined the effects of adiponectin administered by mini-Alzet pumps [ ] and was used as a proof of concept that adiponectin overexpressed in this manner would be effective.

Techniques: Expressing, Transgenic Assay